
pBRIT TAP逆病毒载体质粒图谱序列抗性
产品名称: pBRIT TAP逆病毒载体质粒图谱序列抗性
英文名称: pBRIT TAP逆病毒载体质粒图谱序列抗性说明书价格报价
产品编号: Biovector105802
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
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pBRIT TAP
(Plasmid #17520)
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PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PRICE$65 (USD)
Add to Cart
Available to Academic and Nonprofits Only
BACKBONE
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Vector backbonepBRIT-LoxP-TAP-puro
- Backbone size (bp)5200
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Vector typeMammalian Expression, Retroviral, Cre/Lox
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Selectable markersPuromycin
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
- Tag / Fusion Protein
- TAP (C terminal on backbone)
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ cloning siteSee map (not destroyed)
- 3′ cloning siteSee map (not destroyed)
- 5′ sequencing primerna (Common Sequencing Primers)
RESOURCE INFORMATION
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Terms and Licenses
DEPOSITOR COMMENTS
TAP tag is 6xHis-TEV-3xFLAG (note that two of the FLAG tags are not canonical--the amino acid sequence of the 3xFLAG region is: DYKDHDGDYKDHDIDYKDDDDK)
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBRIT TAP was a gift from Michael Rudnicki (Addgene plasmid # 17520) -
For your References section:
Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84.10.1038/ncb1671 PubMed 18066051